Summary: Use blood to coagulate experiment, blood coagulates restrain experiment and virus to counteract an experiment to be opposite new city epidemic disease (Newcastle Disease, ND) different diseased or infected plant (Lasota and KM-1) the serological relation between undertook study. The blood of detached virus coagulates character can be avoided high by Lasota serum, KM-1 avoids serum place high to restrain, and the value kissing a predestined relationship of two poisonous individual plant is 71% , the result makes clear both for same serum model. The experiment is counteracted in virus in, component of the antigen between Lasota diseased or infected plant and KM-1 poisonous individual plant is difference.

Keyword: Columbine; New city epidemic disease is poisonous; Blood coagulates restrain an experiment; HA price; HI price; Virus counteracts an experiment

The graph classifies date in: 858.39 document label piles up: A article number: 1671-7236 (2005) 00

Epidemic disease of columbine new city is by birds deputy the contagion of a kind of acute that sticks virus to cause, columbine group often come on suddenly, grow like a weed, its are clinical epidemic disease of new city of symptom and chicken is similar, blame with nerve of be addicted to fast hairstyle sees for how, incidence of a disease and mortality are very tall, menace of columbine to raising course of study is very big, what often can cause epidemic disease of gallinaceous new city at the same time is popular, seventies of end global craze is to erupt above all namely epidemic disease of columbine new city, cause worldwide inside the craze of epidemic disease of gallinaceous new city [1] . Our country discovers the earliest 1986 at Hong Kong columbine field, later home also from time to tome the report of epidemic disease of columbine new city, subsequently, shenzhen, Zhuhai moves plant quarantine bureau to be planted from the entrance for many times columbine in the virus that depart gets this disease, area of the Shenzhen at the beginning of 1986 some columbine field erupted this are ill, mortality amounts to 24% [2] . A lot of countries and area depart in succession an a variety of poisonous individual plant, classics appraisal is Ⅰ model deputy stick virus (Paramyxovirus-Type 1, PMI- Ⅰ ) . Microbial lab depart goes to institute of science and technology of animal of Yunnan agriculture college one ND poisonous individual plant (KM-1) , to make clear the serological relation between poisonous individual plant and this ND Lasota poisonous individual plant, serological to virus of two individual plant relation undertook study.

1 material and method

1.1 material

1.1.1 kind of poison: Individual plant of gallinaceous ND Lasota is bought from Chinese animal drug censorial place, individual plant of carrier pigeon ND KM-1 is poisonous individual plant of Yunnan Kunming depart, the syndrome that decrease an egg (Egg Drop Syndrome, EDS) AV-127 is international standard poisonous individual plant, EDS HS-1 is Guizhou depart poisonous individual plant.
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